we're all cringe here

[socksuke_uchiha]

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Comments

Re: Virologist

[(Anonymous)]

what does your typical day entail?

Re: Virologist

[(Anonymous)]

It varies a LOT. The way it works is the week prior our planner will give us all the orders that have been made, and then we have four or five campaigns running at the same time. We also keep up the cell cultures ourselves.

I usually get in at 9, sit around at my desk and drink my coffee, check my email, then we have our little every other day team briefing for 15mins, then we're turned loose.

I typically start the most complex thing first, so I might be extracting DNA from samples for PCR or electroporating/transfecting (electrically shocking cells so they absorb a foreign piece of DNA) which takes a couple of hours. Then I break for lunch, then I start maintaining our cell colonies, which feels a lot like taking care of chickens every day and collecting the eggs (I have experienced both). Every other day I measure the number of cells in our liquid cultures and then "pass" them by splitting them into new bottles and adding more media to dilute the amount of cells in the bottle and give them more space to grow. If we don't do this, they overcrowd and die.

So a lot of colonies just get maintained long before we infect, which is usually the last phase. Then a few times a month we have harvests where we (usually) centrifuge whatever sample down and collect the antigens that were in the cultures and hand them over to analytics.

On really intense days, a couple of times a year, we will handle 2000+ eggs by hand and infect and harvest them in sets of 36, which can take 2-4 hours. It's surprisingly demanding because it gets very very hot under our surgical gowns, bonnets, glasses, gloves, shoe covers, and completely non breathable Tyvek arm covers.